RESUMO
Effects were assessed of the dilutants TRIS and ACP - 101c® with the addition of different guinea fowl (Numida meleagris) egg yolk concentrations. Fifteen ejaculates were collected from five goats of the Anglo Nubian breed. The ejaculates were pooled and then divided into 12 groups, two control groups (GC1 TRIS, with 2.5% Gallus gallus domesticus hen egg yolk GOGD), (GC2 Control Group ACP - 101c®, with the addition of 2.5% Gallus gallus domesticus hen egg yolk GOGD) and ten experimental groups (EG), containing TRIS and ACP added with different concentrations of egg yolk from guinea hen (Numida meleagris) (TRIS 2,5% GONM; TRIS 5% GONM; TRIS 10% GONM; TRIS 15% GONM; TRIS 20% GONM; ACP® 2,5% GONM; ACP® 5% GONM; ACP® 10% GONM; ACP® 15% GONM; ACP® 20% GONM). Then cryopreservation was carried out and the samples stored in liquid nitrogen (-196 °C). After seven days, the samples were thawed and assessed for spermatic kinetics, immunofluorescence and sperm morphology. Analysis of GOMN by the CASA system showed that the various parameters were similar to those of GOGD (P>0.05). The membrane integrity, mitochondrial potential and the acrosome were not influenced by the treatment (P>0.05) nor by the dilutant used for cryopreservation (P>0.05). The spermatic morphology was also preserved by the different GOGD and GONM concentrations in the ACP® and TRIS dilutants, with no statistically significant differences (P<0.05). It was concluded that Numida meleagris egg yolk, as external membrane cryoproctant added to the dilutants ACP-101c® and TRIS, improved goat semen quality.
RESUMO
Among the diseases that affect the reproductive system of domestic animals, brucellosis in the sheep species is important because it generates significant economic losses to sheep rearing. Thus, it is a threat to the growth and productivity of sheep herds. In the face of this problem, the objective of the present research was to identify the prevalence of ovine brucellosis in herds in municipalities of the Teresina, Piauí, Brazil microregion by using the agar gel immunodiffusion assay (AGID) and indirect enzyme-linked immunosorbent assay (ELISA) serological tests. Fourteen municipalities were included in the research. Blood samples were collected from 521 pubescent animals by puncturing the jugular vein. After collection, the samples were submitted to the serological techniques, AGID and indirect ELISA, to detect anti B. ovis antibody. Of the 521 samples submitted to the AGIDtest, 23 (4.41%) were sera reagent and 498 (95.58%) negative. The indirect ELISA tests, 24 (4.61%) suspect samples and 497 (95.39%) negative samples were obtained, and there were no reagent animals in this test, only suspect. The seroprevalence of ovine brucellosis in the Teresina, Piauí microregion was 4.41%. Thus, it is possible to identify sheep with reagent serology to infection by B. ovis, that is present in municipalities in the state of Piauí, Brazil. Furthermore, AGIDwas shown to be more sensitive in detecting animals that had had contact with the etiological agent of the disease.(AU)
Dentre as enfermidades que acometem o sistema reprodutivo dos animais domésticos, a brucelose na espécie ovina tem se destacado por gerar prejuízos econômicos significativos à ovinocultura. Dessa forma, apresenta-se como uma ameaça ao crescimento e à produtividade dos rebanhos ovinos. Diante de tal problemática, objetivou-se, por meio desta pesquisa, identificar a prevalência de brucelose ovina em rebanhos pertencentes a municípios da microrregião de Teresina, Piauí, por meio dos testes sorológicos, imunodifusão em gel de ágar (IDGA) e ensaio imunoenzimático (ELISA) indireto. Quatorze municípios foram incluídos na pesquisa. Para sua execução, colheram-se, por punção venosa da jugular, amostras sanguíneas de 521 animais púberes. Após colheita, as amostras foram submetidas às técnicas sorológicas, IDGA e ELISA indireto, para a detecção de anticorpos anti-B. ovis. Das 521 amostras submetidas ao teste de IDGA, 23 (4,41%) foram sororreagentes e 498 (95,58%) negativas. Quanto ao teste ELISA indireto, obtiveram-se 24 (4,61%) amostras suspeitas e 497 (95,39%) amostras negativas, não havendo animais reagentes neste teste, apenas suspeitos. A soroprevalência da brucelose ovina na microrregião homogênea de Teresina, Piauí, foi de 4,41%. Assim, foi possível identificar ovinos com sorologia reagente à infecção por B. ovis, presente em municípios do estado do Piauí. Além disso, a IDGA mostrou-se mais sensível em detectar animais que tiveram contato com o agente etiológico da doença.(AU)
